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TrueLAMP™

The only LAMP kit that prevents nonspecific amplification and false positives

TrueLAMP™

Suppresses primer-driven nonspecific amplification — so your no-template controls stay negative. 

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A proprietary inhibitor formulated with the polymerase blocks template-free amplification while preserving your true target. No differential time-to-threshold (DTT) analysis needed.

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Read the science (bioRxiv preprint):

“Suppressing Primer-Driven Nonspecific Amplification in LAMP Using TrueLAMP.” 

 

TL8-Strip-Edit_edited.jpg

Available today with colorimetric readout in 10 µL reactions.

 

Fluorescence TrueLAMP™ — coming soon.

THE FALSE POSITIVE PROBLEM

Are you frustrated by false positives in your LAMP assays?

With TrueLAMP™, you can finally trust your LAMP assay results — no amplification in no-template control (NTC), no spurious signal, no wasted runs.

With conventional LAMP

Nonspecific primer amplification turns negatives yellow — and the troubleshooting begins.

❌ Getting amplification in no-template controls (NTCs)

❌ Replacing LAMP reagents trying to find “contamination”

❌ Running LAMP assays in separate rooms

❌ Redesigning LAMP primers again and again

With TrueLAMP™

A proprietary inhibitor blocks nonspecific primer amplification, so only your true target amplifies.

✅ Infectious, inherited diseases, and cancers

✅ Pathogens in crops and food products

✅ Harmful microorganisms in the environment

✅ Pathogens in livestock and pets

HOW IT WORKS

Three steps from sample to a clear answer

1. MIX

Add 1 µL sample (DNA/RNA, or water-suspended cells, bacteria or virions — no extraction needed) to 9 µL master mix (with your LAMP primers) — a 10 µL reaction.

2. Incubate

60 °C for 10 min, then 65 °C for 30–80 min — digital oven, submerged water bath, smart mug or heated-lid thermocycler.

3. Read

Read by eye or quantify photos with a smartphone colorimeter app. Readout:

 

Red = Negative, Yellow = Positive

APPLICATIONS

Build reliable LAMP-based tests for

TrueLAMP™ enables you to develop reliable LAMP-based testing and diagnostics across human, agricultural, environmental and veterinary use.

Human health

Infectious, inherited diseases, and cancers.

Crops & food

Pathogens in crops and food products.

Environment

Harmful microorganisms in the environment.

Animal health

Pathogens in livestock and pets.

PRODUCTS

Choose your TrueLAMP™ format

Two formats, same TrueLAMP™ chemistry and 10 µL protocol. Pick predispensed, ambient-stable convenience — or liquid reagents for custom, scalable workflows.

FORMAT

What's inside

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Storage

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Shipping

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Setup

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LAMP primers

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RT for RNA targets

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Readout

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Best for

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Sizes

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Manual

Dry 8-Strip

Dried polymerase in each tube, dried TrueLAMP™ components in caps, plus 2x 8-Strip buffer

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−20 to 22 °C (>6 months at 22 °C; tolerates 60 °C for 8 days)

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No cold packs · US 2–3 days $30 · Canada $80 · other $120

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Predispensed — add buffer, primers and sample

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User-supplied

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User-supplied RT required (e.g. 0.3 U WarmStart RTx per 10 µL) — dried polymerase has no RT activity

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Color (red → yellow)

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Point-of-care / decentralized assay development

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16 · 160 · 800 reactions

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TrueLAMP™ 8-Strip Kit instruction manual (PDF)

Liquid Kit

2x TrueLAMP™ buffer + liquid polymerase (stored separately); use your own 0.2 mL tubes

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−20 °C (thawed buffer OK at 22 °C up to 1 week)

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Cold, 2–3 business days · US $60 · Canada $200 · other $260

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Mix buffer + polymerase fresh; use within 30 min

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User-supplied

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Not needed — liquid polymerase has RT activity built in

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Color (red → yellow)

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Custom reaction design and scalable lab workflows

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88 · 264 · 1,320 reactions

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TrueLAMP™ Liquid Kit instruction manual (PDF)

Quick order

More from Boltii: cell viability (AquaBluer™) and nucleic acid extraction (AquaGenomic™, AquaRNA™) reagents, plus companion reagents.
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