Selected Publications Using AquaRNA™ / AquaStool™.
- Non-invasive genotyping of transgenic animals using fecal DNA. Chen Z, Mantha RR, Chen JS, Slivano OJ, Takahashi H. Lab Anim (NY). 2012 Mar 20;41(4):102-7. doi: 10.1038/laban0412-102.
PMID: 22430476
Here the authors describe a cost-effective, non-toxic method for genotyping transgenic animals by using the reagent AquaStool to extract fecal DNA and remove PCR inhibitors.
- Method Validation for Extraction of DNA from Human Stool Samples for Downstream Microbiome Analysis. Neuberger-Castillo L, Hamot G, Marchese M, Sanchez I, Ammerlaan W, Betsou F. Biopreserv Biobank. 2020 Apr;18(2):102-116. doi: 10.1089/bio.2019.0112. Epub 2020 Jan 30.
PMID: 31999474
Seven tested stabilizing solutions (OMNIgene( ) GUT, RNAlater( ), AquaStool , RNAssist, PerkinElmer SEB lysis buffer, and DNA Genotek's CP-150) were all compatible with the chemagic MSM I and showed no significant difference in microbiome alpha diversity.
- High-throughput 16S rRNA gene sequencing reveals alterations of intestinal microbiota in myalgic encephalomyelitis/chronic fatigue syndrome patients. Frémont M, Coomans D, Massart S, De Meirleir K. Anaerobe. 2013 Aug;22:50-6. doi: 10.1016/j.anaerobe.2013.06.002. Epub 2013 Jun 19.
PMID: 23791918 Free article. Clinical Trial.
Intestinal dysfunction is a frequent complaint in myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) patients, and previous reports suggest that dysbiosis, i.e. the overgrowth of abnormal populations of bacteria in the gut.
